DNA

Part:BBa_K1896009:Design

Designed by: Bob Van Hove   Group: iGEM16_UGent_Belgium   (2016-10-13)


BsaI MCS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 16
    Illegal BsaI.rc site found at 5


Design Notes

The spacer inbetween the diverging BsaI sites was designed for minimal secondary structure to allow this part to be built from annealed oligonucleotides.


Source

Fully synthetic.

References